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. Author manuscript; available in PMC: 2014 Apr 26.

Published in final edited form as: J Hypertens. 2013 Nov;31(11):2270–2281. doi: 10.1097/HJH.0b013e3283649b33

(a-d) Around 0.6 mL of barium sulfate (0.1mg/mL) was infused in the infra renal aorta through a PE10 tube to visualize renal vascular architecture. The yellow arrows represent interlobular arteries and red arrows represent arcuate arteries. Bar diagram (e) represents the mean percent change ± SEM (n=6/group). Values were obtained after background subtraction and plotted using WT + saline as control. Bar diagram (f) represents the mean percentage change of interlobular artery against the background ± SEM. Bar diagram (g) represents mean percent change of arcuate artery against the background ± SEM. An example of image analysis technique (VesSeg software) using WT+ Saline image is shown in ‘(h)’. TV: Total vascularity; ILA: Interlobular artery; AA: Arcuate artery. *p < 0.05, vs. WT + Ang-II; †P < 0.05 vs. WT and TIMP2^-/- given saline treatment.

(a-d) Around 0.6 mL of barium sulfate (0.1mg/mL) was infused in the infra renal aorta through a PE10 tube to visualize renal vascular architecture. The yellow arrows represent interlobular arteries and red arrows represent arcuate arteries. Bar diagram (e) represents the mean percent change ± SEM (n=6/group). Values were obtained after background subtraction and plotted using WT + saline as control. Bar diagram (f) represents the mean percentage change of interlobular artery against the background ± SEM. Bar diagram (g) represents mean percent change of arcuate artery against the background ± SEM. An example of image analysis technique (VesSeg software) using WT+ Saline image is shown in ‘(h)’. TV: Total vascularity; ILA: Interlobular artery; AA: Arcuate artery. *p < 0.05, vs. WT + Ang-II; †P < 0.05 vs. WT and TIMP2^-/- given saline treatment.

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