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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Rapid Commun Mass Spectrom. 2014 May 15;28(9):1051–1060. doi: 10.1002/rcm.6875

Figure 1.

Figure 1

Example of IM separation improving the quantitative analysis of (a-d) BSA tryptic peptide HLVDEPQNLIK and (e-h) transferrin tryptic peptide NYELLCGDNTRK. In each case, MS/MS without IM separation produced chimeric reporter spectra approaching unity (b, f). After IM separation (c, g), reporter intensities were extracted at drift times of the precursors to produced corrected reporter spectra (d, h). The theoretical ratios for mammalian and yeast peptides were 5:1:5:1 and 1:5:1:5, respectively. IM traces were normalized to their own maximums, and all displayed reporter ratios were obtained from a purity correction algorithm applied to the centroid raw data.