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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Rapid Commun Mass Spectrom. 2014 May 15;28(9):1051–1060. doi: 10.1002/rcm.6875

Figure 2.

Figure 2

IM was used to separate interfering precursors of the same charge (z = +4), tryptic BSA peptide QEPERNECFLSHK and a yeast peptide. MS/MS without IM produced a chimeric reporter spectra approaching unity (b). After IM separation (c), reporter intensities were extracted at drift times of the precursors to produce improved quantitative reporter ion spectra (d). The theoretical ratios for mammalian and yeast peptides were 5:1:5:1 and 1:5:1:5, respectively. IM traces were normalized to their own maximums, and all displayed reporter ratios were obtained from a purity correction algorithm applied to the centroid raw data.