Table 1.
This table summarizes the examples of isobaric interference mitigation by IM. MS/MS and IM-MS/MS refer to purity-corrected ratios without and with IM separation, respectively. The “Log2 Threshold” refers to whether or not a ratio is greater than 1.5 on a Log2 scale, a common threshold for determining up-regulation.
| Mammalian peptides | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Target (m/z, z) | Interferant (m/z, z) | 115/116 Ratio | Log2 Threshold Met? | 117/118 Ratio | Log2 Threshold Met? | ||||||
| Theoretical | MS/MS | IM-MS/MS | MS/MS | IM-MS/MS | Theoretical | MS/MS | IM-MS/MS | MS/MS | IM-MS/MS | ||
| 532.67, 3 | 531.87, 2 | 5.0 | 1.0 | 5.7 | no (0.0) | yes (2.5) | 5.0 | 0.9 | 5.1 | no (−0.2) | yes (2.4) |
| 591.66, 3 | 589.88, 2 | 5.0 | 0.6 | 2.5 | no (−0.7) | no (1.3) | 5.0 | 1.0 | 4.9 | no (0.0) | yes (2.3) |
| 491.76, 4 | 492.26, 4 | 5.0 | 1.3 | 4.4 | no (0.4) | yes (2.1) | 5.0 | 1.3 | 5.7 | no (0.4) | yes (2.5) |
| 499.32, 3 | 499.36, 2 | 5.0 | 0.9 | 3.1 | no (−0.2) | yes (1.6) | 5.0 | 0.8 | 4.3 | no (−0.3) | yes (2.1) |
| Yeast peptides | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Target (m/z, z) | Interferant (m/z, z) | 116/115 Ratio | Log2 Threshold Met? | 118/117 Ratio | Log2 Threshold Met? | ||||||
| Theoretical | MS/MS | IM-MS/MS | MS/MS | IM-MS/MS | Theoretical | MS/MS | IM-MS/MS | MS/MS | IM-MS/MS | ||
| 531.87, 2 | 532.67, 3 | 5.0 | 1.0 | 3.3 | no (0.0) | yes (1.7) | 5.0 | 1.0 | 4.2 | no (0.0) | yes (2.1) |
| 589.88, 2 | 591.66, 3 | 5.0 | 1.7 | 3.7 | no (0.8) | yes (1.9) | 5.0 | 1.0 | 3.2 | no (0.0) | yes (1.7) |
| 492.26, 4 | 491.76, 4 | 5.0 | 0.7 | 4.9 | no (−0.5) | yes (2.3) | 5.0 | 0.8 | 4.0 | no (−0.3) | yes (2.0) |
| 499.36, 2 | 499.32, 3 | 5.0 | 1.1 | 3.5 | no (0.1) | yes (1.8) | 5.0 | 1.3 | 5.0 | no (0.4) | yes (2.3) |