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. 2014 Feb 27;77(4):910–916. doi: 10.1021/np4009865

Figure 3.

Figure 3

PD1n-3 DPA (5) displays potent anti-inflammatory and pro-resolving actions. PD1n-3 DPA, (5), PD1 (3), or vehicle (saline containing 0.01% EtOH) were administered iv 5 min prior to ip administration of zymosan (1 mg). Exudates were collected at 4 h, and the number of infiltrated neutrophils was determined by flow cytometry (top right inset) and light microscopy. Results are mean ± SEM. n = 4 mice per treatment (**p < 0.01 vs vehicle group). (B, C) Macrophages were incubated with vehicle (0.1% EtOH in PBS), PD1n-3 DPA (5) (100 nM to 10 pM), or PD1 (3) (100 nM to 10 pM; 15 min, 37 °C, pH = 7.45) prior to addition of (B) fluorescently labeled zymosan (1:10 macrophages to zymosan) or (C) fluorescently labeled apoptotic human neutrophils. After 60 min (37 °C, pH = 7.45), the incubation was stopped, extracellular fluorescence quenched using trypan blue, and phagocytosis assessed using a SpectraMax M3 plate reader. Results are mean ± SEM. n = 4 macrophage preparations (*p < 0.05 vs PBS-incubated macrophages).