Fig. 7.

Iba1- and GFAP-positive cells located distally to plaques increase in size following MRIgFUS treatment. A, Iba1-positive (green) and GFAP-positive (blue) cell volume surrounding plaques (red) were detected by immunohistochemistry. B, Optical sections were obtained using confocal microscopy and used to create three-dimensional reconstructions. C, Aβ plaque volumes were determined using the surface function and then regions of interest (ROIs) were created, one in contact with the plaque (proximal ROI, dark grey) and second, further from the plaque (distal ROI, light grey). The volume of Iba1-positive cells (D) and GFAP-positive cells (E) were significantly increased in the distal ROI of the MRIgFUS-targeted cortex, compared to the untreated cortex. Similarly, the surface area of microglia (G) and astrocytes (H) were also increased in the distal ROI following MRIgFUS treatment. Scale bar: C = 30μm. Mean ± SEM shown, *p<0.05, **p<0.01.