Fig. 9.

Aβ internalization by Iba1- and GFAP-positive cells is increased in MRIgFUS-treated cortex. Brain sections were stained for Aβ (red), Iba1 (green) and GFAP (blue). Orthogonal views of confocal imaging show Aβ within Iba1-positive (A, representative Iba1-Aβ co-localization encircled) and GFAP-positive cells (B, representative GFAP-Aβ co-localization encircled) indicating internalization of Aβ at 4 days following MRIgFUS treatment. Imaris software was used to create three-dimensional surfaces of glial cells and detect Aβ within the glia found proximal (Aβ in red) and distal (Aβ in purple) to plaques (C, Aβ within a single cell shown in D). E, Aβ counts within Iba1-positive cells was greater within MRIgFUS-treated cortex in both proximal and distal regions surrounding plaques. F, Similarly, GFAP-positive cells within the MRIgFUS-treated cortex also contained a greater number of Aβ counts than those in the untreated, contralateral cortex. In addition, the total volume of internalized Aβ was greater in Iba1-positive (G) and GFAP-positive (H) cells within MRIgFUS-treated regions, proximal and distal to plaques. Scale bar: A–B = 25 μm. Mean ± SEM shown, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.