Table 1. Details of the primer pairs used to amplify the exons of the CHM gene.
| Exon | Direction | Primer | Amplicon (bp) | Tm (°C) | |
|---|---|---|---|---|---|
| 1 |
* |
FWD |
GACCTTCCACCCAAGAACTAC |
216 |
55.0 |
| REV |
ACAGTCTTCCTAAACTTTGTCC |
52.4 |
|||
| 2 |
+ |
FWD |
CAGCAATGGCATGTATTGAACATT |
384 |
54.6 |
| REV |
TGATGCATTTGGTTTATTCTCAAAGA |
53.6 |
|||
| 3 |
Δ |
FWD |
AATTGTTACAGAAGAAGCTACTATGG |
160 |
52.9 |
| REV |
GGTTTTCTTCAGTGCAGGGTTA |
55.3 |
|||
| 4 |
Δ |
FWD |
TTTTTCTCCCCTTCCTTTGA |
411 |
51.5 |
| REV |
TGTAGCAACCAATGCCACAT |
55.1 |
|||
| 5 |
Δ |
FWD |
TTCCAATACAGTTCCCCTGT |
648 |
53.4 |
| REV |
TGTTTTCAATGCAAAGATGG |
49.4 |
|||
| 6 |
Δ |
FWD |
ATGGATCAGGTTTTGCTGCT |
481 |
54.9 |
| REV |
ACCACGGAGGACTGGAATTT |
56.3 |
|||
| 7 |
Δ |
FWD |
GGGAAAAAAGTGTAATTTGG |
330 |
47.4 |
| REV |
TGGCTAACCTATTGATAGTGC |
52.0 |
|||
| 8 |
Δ |
FWD |
TGTCCAAAATGTAATACCACC |
402 |
50.5 |
| REV |
TCAAGTATCACTTTTAGAAGGG |
49.7 |
|||
| 9 |
Δ |
FWD |
TCTGGTTTGCTCACAGTTCT |
346 |
54.0 |
| REV |
TGAAGGTTACTTATATCATCCTTAC |
49.9 |
|||
| 10 |
Δ |
FWD |
GCCCTCAAAATAGCAACAAGA |
452 |
53.4 |
| REV |
GGCTTCCCTAAAACCAGACC |
55.6 |
|||
| 11 |
Δ |
FWD |
GGGAGGTGACACTTTTATCC |
500 |
52.8 |
| REV |
AGAGAGTAAAACGGTGCTTG |
52.6 |
|||
| 12 |
Δ |
FWD |
AAATATGTTTCAAATTCTGTTCCAAA |
436 |
50.6 |
| REV |
AAGGGGATGGTGTGAAATGA |
54.0 |
|||
| 13 |
* |
FWD |
CGCTCAGCTCTCTATTATCCAT |
257 |
53.8 |
| REV |
CCGGAAGATTATGATGGTTACAT |
52.5 |
|||
| 14 |
* |
FWD |
TAGGCTACACAGTGTAGTAA |
322 |
49.6 |
| REV |
GACTTCTCTCCTCCCAGAGG |
56.1 |
|||
| 15 |
Δ |
FWD |
TGAGGTACTGCCATCCTTGA |
478 |
53.9 |
| REV | TGAGACCAGTCAGAATTTCCAA | 55.5 |