Table 1. Sequences of RT-PCR primers used in the present study.
| Gene | Species | Primer sequence (5’-3’) | Product size (bp) |
|---|---|---|---|
| HFE |
Mouse |
F:GGCTTCTGGAGATATGGTTAT |
142 |
| R:GACTCCACTGATGATTCCGATA |
|||
| Hepcidin |
Mouse |
F:GCACCACCTATCTCCATCAACAGA |
188 |
| R:GGTCAGGATGTGGCTCTAGGCTAT |
|||
| Hemojuvelin |
Mouse |
F:GGCTGAGGTGGACAATCTTC |
551 |
| R:GAACAAAGAGGGCCGAAAG |
|||
| TfR1 |
Mouse |
F:GCCCAAGTATTCTCAGATATGAT |
633 |
| R:TAGAAGTAGCACGGAAGTAGTCTC |
|||
| Matriptase-2 |
Mouse |
F:CCCTCTCTGGACTACGGCTTGGC |
842 |
| R:CGGCTCACCTTGAAGGACAC |
|||
| Neogenin |
Mouse |
F:TCGAACATTCACTCCGTTTTA |
621 |
| R:CGCATCAGAAATACCAAGTCTAC |