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. Author manuscript; available in PMC: 2014 Apr 27.
Published in final edited form as: Biochemistry. 2011 Apr 11;50(17):3502–3511. doi: 10.1021/bi200239k

Figure 2.

Figure 2

(A) Mass spectrum of intact AFMO complex by native electrospray mass spectrometry. Nano-ESI conditions: voltage of ISCID: 180 V; CID: 20 eV. The vertical blue lines are theoretical m/z values for charge states +20 to +24 generated using the labeled MW; the +23 charge state was assigned to the base peak. (B) Gaussian functions with bandwidth of m/z = 6 were used to simulate the various resolved peaks (+22 and +23) of ions with molecular weights of 139,043 Da (pink), 139,685 Da (green), 140,329 Da (blue), and 140,976 Da (red).