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(A) The BChl a isolated from the FMO protein has a monoisotopic mass of 910.5 Da. No fragmentation occurs under normal ESI conditions with gentle desolvation. (B) BChl a is significantly fragmented under harsher desolvation conditions similar to that used in the native electrospray of the AFMO complex. The dominant ion of m/z = 632.2 is the fragment of the BChl a without the phytyl tail (i.e., protonated BChlide a).
