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. Author manuscript; available in PMC: 2015 Apr 28.
Published in final edited form as: J Control Release. 2014 Feb 15;180:51–59. doi: 10.1016/j.jconrel.2014.02.005

Fig. 5.

Fig. 5

Validation of the diaCEST approach using fluorescence imaging and histology. (A) Representative fluorescence images and average fluorescence intensity (measured as radiant efficiency) of tumors harvested from the TNF-α control group (n=3), the Liposome group (n=5) and the Liposome+TNF-α group (n=5). (*, P<0.05; **, P<0.01, one-way ANOVA). (B) Fluorescence histology of excised tumors showing the relative accumulation and distribution of rhodamine-labeled diaCEST liposomes (red, yellow arrows) for the Liposome group and the Liposome+TNF-α group. Nuclei are stained with DAPI (blue). (C) CD31 staining shows distribution of blood vessels (black arrows) in sections of similar regions in (B). Arrows indicate representative regions (Scale bar=100 µm).