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Enhancer activity of KO-H3K4me1 sites. (A) H3K27ac ChIPs at KO-H3K4me1 sites of neuronal lineage genes in KO MEFs, WT MEFs, KO iPS cells, and WT iPS cells before and after 4 d of RA treatment. (B) Luciferase activity to assess reporter activity after subcloning of DNA fragments at KO-H3K4me1 sites using Lsh KO MEFs, HeLa, and neuronal cell line N2A. pGL-3P (promoter) served as a negative control.
