Table 1.

Effect of phage mutations on phage and SaPI titers

Donor strain			Titer
Phage	SaPI	pCN51*	Phage†	SaPI‡
ϕ11	—	—	6.6 × 109	—
ϕ11 ΔterS	—	—	<10	—
ϕ11	SaPIbov1	—	2 × 107	1 × 107
ϕ11	SaPIbov5	—	2 × 109	1.3 × 107
ϕ11	SaPIbov5 Δcos	—	1.6 × 109	1.1 × 107
ϕ11 ΔterS	SaPIbov1	—	<10	1.9 × 107
ϕ11 ΔterS	SaPIbov5	—	<10	<10
ϕ11 ΔterS	SaPIbov5	pCN51-terSϕ11	1.8 × 105	2.3 × 105
80α	—	—	8 × 1010	—
80α	SaPIbov1	—	3.4 × 109	1 × 107
80α	SaPIbov5	—	1.5 × 1011	1.2 × 107
ϕ12	—	—	8 × 107	—
ϕ12 Δhnh	—	—	<10	—
ϕ12 Δhnh	—	pCN51-hnhϕ12	2.2 × 104	—
ϕ12	SaPIbov1	—	1.1 × 109	<10
ϕ12	SaPIbov5	—	1.1 × 106	1.1 × 106
ϕ12	SaPIbov5 Δcos	—	1.4 × 107	<10
ϕ12 Δhnh	SaPIbov5	—	<10	<10
ϕ12 Δhnh	SaPIbov5	pCN51-hnhϕ12	1.4 × 104	8.1 × 104
ϕSLT	—	—	6.2 × 106	—
ϕSLT Δhnh	—	—	<10	—
ϕSLT Δhnh	—	pCN51-hnhϕSLT	3.1 × 104	—
ϕSLT	SaPIbov1	—	2.7 × 106	<10
ϕSLT	SaPIbov5	—	8.2 × 105	8.7 × 103
ϕSLT	SaPIbov5 Δcos	—	3.3 × 106	<10
ϕSLT Δhnh	SaPIbov5	—	<10	<10
ϕSLT Δhnh	SaPIbov5	pCN51-hnhϕSLT	5.7 × 103	1.3 × 103

The means of results from three independent experiments are shown. Variation was within ±5% in all cases. —, the strain has not SaPIs or plasmids, and consequently, the transfer of the SaPIs can not be analyzed.

^*Complemented both the donor and the recipient strains.

^†PFU/mL induced culture, using RN4220 as the recipient strain.

^‡Number of transductants per milliliter induced culture, using RN4220 as the recipient strain.