Fig. 2.

The last 97 amino acids of Hst3 comprise a damage-responsive degron. (A) Hst3 full length or lacking the last 97 amino acids was expressed under the control of the GAL1 promoter and tagged with GFP. Hst3 was induced by the addition of galactose, and turnover was followed for 45 min, with time points taken every 15 min. For turnover in 0.2 M HU, cells were grown in galactose for 2.5 h, 0.2 M HU was added for an additional 2.5 h, and then cells were treated with cycloheximide. (B) GFP or GFP fused to either full-length Hst3 or to the last 97 amino acids of Hst3 was expressed under the control of the GAL1 promoter. The experiment was performed as in A.