Table 2.
Numbers of clonogenic hemopoietic progenitor cells in MplPF4cre/PF4cre and control mice
| Genotype (n) | Blast | G | GM | M | Eo | Meg | Total |
| Bone marrow | |||||||
| Mplfl/fl (6) | 4.5 ± 2.2 | 30 ± 5 | 22 ± 11 | 30 ± 11 | 3.5 ± 1.9 | 21 ± 9 | 111 ± 30 |
| MplPF4cre/PF4cre (6) | 40 ± 15* | 70 ± 55 | 85 ± 30* | 73 ± 54 | 4.8 ± 3.6 | 88 ± 39* | 360 ± 80* |
| TPOTg (4) | 12 ± 4* | 48 ± 20 | 63 ± 17* | 42 ± 10* | 2.8 ± 1.0 | 39 ± 12* | 204 ± 55* |
| Spleen | |||||||
| Mplfl/fl (3) | 1.0 ± 1.0 | 1.0 ± 1.7 | 1.3 ± 2.3 | 3.0 ± 4.4 | 0.0 ± 0.0 | 8.0 ± 8.2 | 14 ± 13 |
| MplPF4cre/PF4cre (3) | 35 ± 18* | 41 ± 10* | 89 ± 49* | 45 ± 19* | 1.3 ± 1.2 | 204 ± 48* | 415 ± 131* |
| TPOTg (2) | 2.0 ± 0.0 | 1.5 ± 0.7 | 1.0 ± 1.4 | 1.0 ± 1.4 | 0.0 ± 0.0 | 24 ± 14 | 29 ± 12 |
Numbers of colonies from 25,000 unfractionated bone marrow cells or 50,000 spleen cells cultured in stem-cell factor (SCF, 100 ng/mL), interleukin-3 (IL-3, 10 ng/mL), and erythropoietin (EPO, 2 U/mL) with the type and number of colonies scored after 7 d. Eo, eosinophil; G, granulocyte; GM, granulocyte-macrophage; M, macrophage; Meg, megakaryocyte. *P < 0.03 compared with Mpl+/+ by Student's unpaired two-tailed t test.