Table 2.
Alterations in gyrA and parC genes detected by MAMA-PCR and confirmed by sequence analysis for selected isolates.
| Total number of mutations | Number of isolates | Alterations detected by MAMA-PCR | Confirmation by sequencing (n) | ||||
|---|---|---|---|---|---|---|---|
| gyrA | parC | ||||||
| 83 | 87 | 80 | 84 | gyrA | parC | ||
| None | 52 | None | None | None | None | Wild type (7) | Wild type (7) |
| 1 | 8 | Mutation | None | None | None | Ser83Tyr (4) | ND |
| 2 | 1 | Mutation | Mutation | None | None | ND | ND |
| 14 | Mutation | None | Mutation | None | Ser83Ile (8) | Ser80Ile (9), Ser80Arg (1) | |
| 2 | Mutation | None | None | Mutation | Ser83Ile (2) | Glu84Lys (2) | |
| 3 | 16 | Mutation | Mutation | Mutation | None | Ser83Phe + Asp87Ala (4) | Ser80Ile (6) |
ND: not done.