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. 2014 Apr 10;2014:686921. doi: 10.1155/2014/686921

Figure 5.

Figure 5

PME increased ALP activity in MC3T3-E1 osteoblast without affecting cell proliferation. (a) Dose-dependent effect of PME on cell proliferation. MC3T3-E1 osteoblasts were treated with 0 (control), 20, 40, 80, 160, and 320 μg/mL of PME for 48 hrs and the cell proliferation was determined by MTT assay. The cell proliferation was expressed as percentage cell viability over the untreated control. (b) Cells were treated with E2 (10 nM), PME (40, 80, and 160 μg/mL), ICI (1 μM) with or without PME (80 μg/mL) for 48 hrs. ALP activity was then measured and results are expressed as percentage over the untreated control. Results are expressed as mean values ± SE of five replicates. *P < 0.05 when compared to untreated control.