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Optimization of the Agrobacterium-mediated transformation based on transient gusA expression on VKD's PLBs. (a) PLB size; (b) wounding; (c) concentration of acetosyringone; (d) cocultivation period; (e) Agrobacterium density; and (f) Immersion period. Results were analysed by one-way ANOVA and means were compared by Tukey's test. Vertical bars represent ± SE of means of 6 replicates. Different letters indicate values which are significantly different (P ≤ 0.05).
