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. 2014 Apr 8;2014:917698. doi: 10.1155/2014/917698

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Copyright © 2014 Daniele Lettieri Barbato et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RSV does not revert inflammatory markers upregulation in ATGL lacking cells. (a) Differentiated 3T3-L1 adipocytes (8-days-old) were transfected with siRNA against ATGL [ATGL(−)] or with a scramble siRNA (Scr) and treated with 100 μM RSV for 24 h. RT-qPCR analysis of relative ATGL, PPARα, TNFα, and IL-6 mRNA levels. Values are given as means ± S.D. ^(a) P < 0.05 versus Scr cells; ^(∗) P < 0.01 versus RSV untreated cells. (b) Western blotting analysis of IκB and phosphoactive form of NFκB (pNFκB) in total protein extracts of adipocytes. β-Actin was used as loading control. Immunoblots are representative of at least 3 independent experiments.