Table 2.
Summary of the mouse lines discussed, with nerve ending descriptions for the neuron types labeled.
| Mouse line # | Mouse line | Sensory cell type labeled or ablated | Peripheral nerve ending (skin) | Central projection (spinal cord) | Original reference(s) |
|---|---|---|---|---|---|
| 1 | TRPV1-i-PLAP-i-nLacZ | Small diameter C fibers and subset of medium diameter Aδ fibers (heat, pain) | Free nerve endings | Laminae I–II and V | Cavanaugh et al., 2011a |
| 2 | TRPV1-Cre | Small diameter C fibers and subset of medium diameter Aδ fibers (heat, pain) | Free nerve endings | Laminae I–II and V | Cavanaugh et al., 2011b; Mishra et al., 2011 |
| 3 | TRPV1-DTR | Small diameter C fibers and subset of medium diameter Aδ fibers (heat, pain) | Free nerve endings | Laminae I–II and V | Pogorzala et al., 2013 |
| 4 | TRPM8-GFP | Small diameter C fibers and subset of medium diameter Aδ fibers (cold, pain) | Free nerve endings | Primarily lamina I | Takashima et al., 2007 |
| 5 | TRPM8-fGFP | Small diameter C fibers and subset of medium diameter Aδ fibers (cold, pain) | Free nerve endings | Primarily lamina I | Dhaka et al., 2008 |
| 6 | TRPM8-DTR | Small diameter C fibers and subset of medium diameter Aδ fibers (cold, pain) | Free nerve endings | Primarily lamina I | Pogorzala et al., 2013 |
| 7 | CGRP-GFP-DTR | Peptidergic small diameter C fibers and subset of medium diameter Aδ fibers (heat, pain) | Free nerve endings | Laminae I–II and V | McCoy et al., 2013 |
| 8 | MrgprD-EGFP | Small diameter C fibers (high threshold mechanical, pain) | Free nerve endings | Lamina II | Zylka et al., 2005 |
| 9 | MrgprD-PLAP | Small diameter C fibers (high threshold mechanical, pain) | Free nerve endings | Lamina II | Zylka et al., 2005 |
| 10 | MrgprD-DTR | Small diameter C fibers (high threshold mechanical, pain) | Free nerve endings | Lamina II | Cavanaugh et al., 2009 |
| 11 | MrgprD-ChR2 | Small diameter C fibers (high threshold mechanical, pain) | Free nerve endings | Lamina II | Wang and Zylka, 2009 |
| 12 | Nav1.8:ChR2 | Small diameter C fibers (all types) | Free nerve endings | Laminae I–II and V | Madisen et al., 2012; Daou et al., 2013 |
| 13 | MrgprA3-EGFP:Cre | Non-peptidergic, small diameter C fibers (itch) | Free nerve endings | Lamina II | Han et al., 2012 |
| 14 | MrgprB4-PLAP | Non-peptidergic, small diameter C fibers (stroking) | Free nerve endings | Lamina II | Liu et al., 2007 |
| 15 | MrgprB4-tdTomato-2A-Cre | Non-peptidergic, small diameter C fibers (stroking) | Free nerve endings | Lamina II | Vrontou et al., 2013 |
| 16 | VGLUT3-EGFP | Non-peptidergic, small diameter C fibers (low threshold mechanical) | Longitudinal lanceolate endings | Lamina II | Seal et al., 2009 |
| 17 | VGLUT3-KO | Non-peptidergic, small diameter C fibers (low threshold mechanical) | Longitudinal lanceolate endings | Lamina II | Seal et al., 2009 |
| 18 | TH-CreER | Non-peptidergic, small diameter C fibers (low threshold mechanical) | Longitudinal lanceolate endings | Lamina II | Li et al., 2011 |
| 19 | TrkB-tauEGFP | Medium diameter Aδ fibers (low threshold mechanical) | Longitudinal lanceolate endings | Laminae II–III | Li et al., 2011 |
| 20 | Npy2r-GFP | large diameter Aβ fibers (low threshold mechanical) | Longitudinal lanceolate endings | Laminae III–V | Li et al., 2011 |
| 21 | Chondrolectin-PLAP | Large diameter Aβ fibers (low threshold mechanical) | Longitudinal lanceolate endings (in mystacial pad) | No data | Sakurai et al., 2013 |
| 22 | Parvalbumin:Cre | Large diameter Aα and Aβ fibers (low threshold mechanical; and muscle tension and contraction) | Muscle spindle, Golgi tendon; Merkel and lanceolate endings (in mystacial pad) | Laminae III–V | Sakurai et al., 2013 |
| 23 | DOR-EGFP | Sub-populations of both non-peptidergic C fibers and myelinated NF200-positive fibers (mechanical pain) | Meissner corpuscles, Merkel, and circumferential endings | Laminae I–V | Scherrer et al., 2009; Bardoni et al., 2014 |
The numbers in the first column are referred to in Figure 4 to show the origin of the data classifying the neuron types. Abbreviations: TRPV1, transient receptor potential cation channel subfamily V member 1; i, IRES; PLAP, placental alkaline phosphatase; nLacZ, nuclear-localized β-galactosidase; Cre, cre recombinase; DTR, diphtheria toxin receptor; TRPM8, transient receptor potential cation channel subfamily M member 8; GFP, green fluorescent protein; fGFP, farnesylated green fluorescent protein; CGRP, calcitonin gene-related peptide; MrgprD, Mas-related G-protein-coupled receptor D; ChR2, channelrhodopsin 2; Nav1.8, voltage-gated sodium channel 1.8; MrgprA3, Mas-related G-protein-coupled receptor A3; MrgprB4, Mas-related G-protein-coupled receptor B4; VGluT3, vesicular glutamate transporter type 3; TH, tyrosine hydroxylase; TrkB, tyrosine receptor kinase B; Npy2r, neuropeptide Y receptor type 2; DOR, delta opioid receptor.