Table 1.
ID | Habitat | Age (weeks after planting) | Treatment | Disease condition | Plant replicates | PCR replicatesb | Sequencing |
---|---|---|---|---|---|---|---|
PYC | Phyllosphere | Young (2) | Untreated (Control) | not determinable | 2 | 2 | MWG, Eurofins |
PYRS | Phyllosphere | Young (2) | R. solani inoculated | not determinable | 2 | 2 | MWG, Eurofins |
PYfzb42RS | Phyllosphere | Young (2) | R. solani and FZB42 inoculated | not determinable | 2 | 2 | MWG, Eurofins |
PMG | Phyllosphere | Mature (4) | Untreated (Control) | healthy | 3 (6a) | 2 | MWG, Eurofins |
PMK | Phyllosphere | Mature (4) | R. solani and FZB42 inoculated | bottom rot | 3 (6a) | 2 | MWG, Eurofins |
RYC | Rhizosphere | Young (2) | Untreated (Control) | not determinable | 2 | 2 | Macrogen, Korea |
RYRS | Rhizosphere | Young (2) | R. solani inoculated | not determinable | 2 | 2 | Macrogen, Korea |
RYfzb42RS | Rhizosphere | Young (2) | R. solani and FZB42 inoculated | not determinable | 2 | 2 | Macrogen, Korea |
RMG | Rhizosphere | Mature (4) | Untreated (Control) | healthy | 3 (6a) | 2 | Macrogen, Korea |
RMK | Rhizosphere | Mature (4) | R. solani and FZB42 inoculated | bottom rot | 3 (6a) | 2 | Macrogen, Korea |
Two Independent DNA extractions per plant were pooled prior to PCR.
PCR was carried out twice for each DNA sample and each PCR step and pooled prior to sequencing.