Table 1.
Sample design description.
| ID | Habitat | Age (weeks after planting) | Treatment | Disease condition | Plant replicates | PCR replicatesb | Sequencing |
|---|---|---|---|---|---|---|---|
| PYC | Phyllosphere | Young (2) | Untreated (Control) | not determinable | 2 | 2 | MWG, Eurofins |
| PYRS | Phyllosphere | Young (2) | R. solani inoculated | not determinable | 2 | 2 | MWG, Eurofins |
| PYfzb42RS | Phyllosphere | Young (2) | R. solani and FZB42 inoculated | not determinable | 2 | 2 | MWG, Eurofins |
| PMG | Phyllosphere | Mature (4) | Untreated (Control) | healthy | 3 (6a) | 2 | MWG, Eurofins |
| PMK | Phyllosphere | Mature (4) | R. solani and FZB42 inoculated | bottom rot | 3 (6a) | 2 | MWG, Eurofins |
| RYC | Rhizosphere | Young (2) | Untreated (Control) | not determinable | 2 | 2 | Macrogen, Korea |
| RYRS | Rhizosphere | Young (2) | R. solani inoculated | not determinable | 2 | 2 | Macrogen, Korea |
| RYfzb42RS | Rhizosphere | Young (2) | R. solani and FZB42 inoculated | not determinable | 2 | 2 | Macrogen, Korea |
| RMG | Rhizosphere | Mature (4) | Untreated (Control) | healthy | 3 (6a) | 2 | Macrogen, Korea |
| RMK | Rhizosphere | Mature (4) | R. solani and FZB42 inoculated | bottom rot | 3 (6a) | 2 | Macrogen, Korea |
Two Independent DNA extractions per plant were pooled prior to PCR.
PCR was carried out twice for each DNA sample and each PCR step and pooled prior to sequencing.