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. 2014 Apr 22;5:148. doi: 10.3389/fpls.2014.00148

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Copyright © 2014 Strohm, Barrett-Wilt and Masson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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arg1-2 toc120-3 and arg1-2 ppi3-1 seedlings showed increased variability of root growth angles compared to WS wild type and arg1-2, toc120-3, and ppi3-1 single mutants (F-test, p < 0.05, and n = 32–44). The seedlings were grown vertically for 3 days. They were then tilted backward 30° and grown for an additional 4 days. The root angles were then measured from the root-hypocotyl junction to the root tip using Adobe Photoshop. These angles were placed into one of six 60° bins, and the corresponding histograms were drawn to the right of each panel. In these histograms, the size of each wedge is proportional to the fraction of plants whose roots grew in the direction of the corresponding bin. This experiment was repeated three independent times with similar results.