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. 2014 Apr 28;4:4821. doi: 10.1038/srep04821

Figure 2. Combined staurosporine and ATRA modulated the protein level of C/EBPβ, C/EBPε, PML-RARα as well as the phosphorylation of MEK and ERK.

Figure 2

Cells were treated with 2 nM staurosporine(ST), 1 μM ATRA(RA) alone and the combined treatment (ST + RA) for 24 hours. The protein level of C/EBPβ and C/EBPε was measured by Western blot analysis using 20 μg protein, while 50 μg protein was loaded for Western blot analysis of the phosphorylation of MEK and ERK1/2. The same membrane incubated with the phosphorylated Erk1/2 or MEK1/2 was stripped and followed by detection of MEK and ERK1/2. For Western blot analysis of PML-RARα, cells were treated with the indicated drugs for 72 hours and 20 μg protein was loaded. Since different amount of protein and diverse time points for collecting protein were used, each has the expression of β-actin as internal control. Similar results were obtained in three independent experiments. The full size blots were shown in the Supplemental Fig. S1 online.