Figure 4. PA1r is LPS negative and prevents phage adsorption.
(A): Agglutination test: PA1 remains single cells in 4% NaCl, but LPS negative bacteria PA1r are agglutinated. (B): Adsorption Assay of Bacteriophage PaP1 to P. aeruginosa PA1, PA1r, PA1ΔgalU and PA1r:galU. Phage adsorption to PA1 and PA1r were significantly different (Tukey's Multiple Comparison Test: P < 0.05). (C): Transmission electron micrograph shows that phage PaP1 binds to PA1 but not to PA1r.