Table 2. Bacterial strains, plasmids, and phage used in this study.
| Bacterial strain or plasmid | Relevant feature(s)a | Reference or source |
|---|---|---|
| P. aeruginosa Strains | ||
| PA1 | Wide type strain for phage PaP1 | [23] |
| PA1r | Phage resistant mutant | This study |
| PA1R-37 | Phage resistant mutant | This study |
| PA1R-57 | Phage resistant mutant | This study |
| PA1R-61 | Phage resistant mutant | This study |
| PA1ΔhmgA | Insertional deletion of hmgA | This study |
| PA1ΔgalU | Insertional deletion of galU | This study |
| PA1r: hmgA | Complementation of hmgA | This study |
| PA1r: galU | Complementation of galU | This study |
| E.coli strains | ||
| S17-1 (λ pir) | Conjugative donor strain | [51] |
| SM10pir | Conjugative donor strain | [28] |
| Bacteriophage | ||
| PaP1 | Lytic phage | [23] |
| Plasmid | ||
| pUCP24 | 4.036-kp pUC18-based broad-host-range vector, Gmr | [52] |
| pEX18Tc | Gene replacement vector, Tcr | [51] |
| pCM639 | ISphoA|hah-Tc delivery plasmid | [28] |
| pucp-galU | Broad-host-range vector containing galU, Gmr | This study |
| pucp-hmgA | Broad-host-range vector containing hmgA, Gmr | This study |
| pEX –galU | galU deletion vector, Tcr | This study |
| pEX -hmgA | hmgA deletion vector, Tcr | This study |
Abbreviations: Gmr, gentamicin resistance. Tcr, tetracycline resistance.