Fig. 5. Inhibition of Xag and Xagr2 mRNA translation by anti-sense morpholinos elicits brain abnormalities similar to those observed when Ras-dva1 and Fgf8 were inhibited.

(A,C) Inhibition of Xag (A) and Xagr2 (C) mRNA translation by anti-sense morpholinos elicits reduction of the telencephalon (see enlarged images in the upper row, black arrow), otic vesicles (yellow arrowhead) and eyes (red arrowhead for Xag downregulation). (A′,C′) Overlays of the white light and fluorescent images of embryos shown in panels A and C demonstrate the distribution of cells containing injected MO mixed with a FLD tracer. (B,D) Rescue of anatomical abnormalities by co-injection of Xag and Xagr2 mRNAs with Xag and Xagr2 morpholinos to these genes. (B′,D′) Overlays of the white light and fluorescent images of embryos shown in panels B and D demonstrate the distribution of cells containing the injected MO mixed with a FLD tracer.