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. 2014 Feb 25;3(3):192–203. doi: 10.1242/bio.20147401

Fig. 6. Agrs, Fgf8 and Ras-dva1 regulate expression of each other.

Fig. 6.

(A–C) Injections of Xag and Xagr2 MO elicit inhibition of FoxG1, Fgf8 and Ras-dva1 expression in the anterior ectoderm of midneurula embryos. (D) Injections of Ras-dva MO elicit inhibition of Fgf8 expression in the anterior ectoderm of midneurula embryos. (E,F) Co-injection of Agr mRNA (equimolar mixture of Xag1, Xag2, Xagr2A and Xagr2B mRNAs) or Ras-dva1 mRNA is unable to prevent the inhibitory influence of Fgf8 MO on FoxG1 expression. (G) Injection of Fgf8a mRNA elicits massive ectopic expression of FoxG1 in the anterior ectoderm. (H) Induction of FoxG1 expression elicited by ectopic Fgf8a is suppressed by co-injection of Agrs MO (a mixture of Xag and Xagr2 MO). (A′–H′) Fluorescent image of the embryo shown in panels A–H demonstrates the distribution of injected cells labeled by the co-injected FLD tracer.