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. 2014 Apr 17;5(4):e1181. doi: 10.1038/cddis.2014.145

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Suppression of caspase-14 as well as mesotrypsin caused a marked increase of nuclear remnants in the cornified layer. (a) Effect of caspase-14 and mesotrypsin suppression. The siRNAs strongly suppressed the expression of these proteases, by suppressed by 90% and 76%, respectively. (b) Skin equivalent models were prepared using keratinocytes treated with nonspecific siRNA (control), siRNA to caspase-14, siRNA to mesotrypsin and combined siRNAs to caspase-14 and mesotrypsin. Images of H&E staining are shown. Scale bars: 50 μm. (c) Quantitative analysis of the presence of nuclear remnants in the cornified layer. Numbers of nuclear remnants in the cornified layer were counted in each 450 μm field for total of 4.5 mm length and expressed as mean±S.D. *P<0.05, **P<0.01, ***P<0.001