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Accumulation of macrophages in primary tumors after different treatments using IHC staining. (a) Detection of F4/80 murine macrophage markers. The arrows indicate macrophage marker F4/80 in tumor lesions. The scale bar of each picture is 50 μm. (b) Quantification and comparison of F4/80 markers in primary tumors in different treatment groups. The data represent the mean±S.D. from three randomly selected tissue sections. *P<0.05
