Figure 2.

PMB treatment reduces pro-inflammatory gene expression following IR injury. Differential expression of cytokines, chemokines, cell adhesion molecules, as well as genes encoding Nos2 and subunits of the reactive oxygen species-producing NAD(P)H oxidase was assessed in sham-operated and ischemic retinas of PMB- and PBS-treated animals, 6 and 12 hours after reperfusion, by using quantitative RT-PCR. For each gene, results are expressed as a percentage of corresponding value in the sham-operated eye of PBS-treated animals (*P < 0.05, **P < 0.01, five animals per group). IR, ischemia reperfusion; PBS, phosphate buffered saline; PMB, polymyxin B.