Figure 1.

Activation Tag Line PGX1^AT Displays Enhanced Hypocotyl Length, Cell Length, and PGX1 Expression.

(A) Hypocotyls of 6-d-old etiolated Arabidopsis Col (wild type, left) and activation tag line PGX1^AT (right) grown on MS media without Suc.

(B) Quantification of hypocotyl length for 2- to 6-d-old etiolated Col and PGX1^AT seedlings (n ≥ 63 seedlings per time point from three independent experiments). DAS, days after sowing.

(C) Cell length at the top, middle, and bottom of hypocotyls of 6-d-old etiolated Col and PGX1^AT seedlings (n ≥ 60 seedlings from three independent experiments).

(D) qPCR quantification of PGX1 mRNA expression levels in 6-d-old etiolated Col and PGX1^AT seedlings. ACT2 was used as an internal control. The expression level of PGX1 in Col was normalized to 1, and the relative expression level of PGX1 in PGX1^AT line was calculated relative to this value. Error bars indicate sd (n = 3 replicates). Asterisks indicate significant differences (P < 0.001, t test).