Figure 2.

Global Overexpression of PGX1 Promotes Hypocotyl Elongation.

(A) Hypocotyls of 6-d-old etiolated Arabidopsis Col and three independent transgenic lines expressing PGX1 driven by the cauliflower mosaic virus 35S promoter (PGX1^OE-1, PGX1^OE-45, and PGX1^OE-48) grown on MS media without Suc.

(B) Quantification of hypocotyl length for 2- to 6-d-old etiolated Col and PGX1^OE seedlings (n ≥ 66 seedlings from three independent experiments).

(C) mRNA expression levels of PGX1 in Col and three PGX1^OE transgenic plants quantified by qPCR. ACT2 was amplified as a control.

(D) Cell length at the top, middle, and bottom of hypocotyls of 6-d-old etiolated seedlings of Col and three PGX1^OE transgenic plants (n ≥ 30 seedlings from at least two independent experiments). Error bars indicate sd.

Asterisks in (B) indicate significant differences between Col and PGX1^OE lines (P < 0.05, t test). Lowercase letters in (C) and (D) indicate significantly different groups (P < 0.05, one-way ANOVA and Tukey test).