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. 2014 Mar 28;26(3):1018–1035. doi: 10.1105/tpc.114.123968

Figure 4.

Figure 4.

PGX1 Complements the Hypocotyl Length Phenotype of pgx1-1 and pgx1-2 Mutants.

(A) RT-PCR analysis of PGX1 expression in Col and lines expressing PGX1-GFP driven by the PGX1 promoter in the pgx1-1 or pgx1-2 background. ACT2 was amplified as an internal control. Total RNA was isolated from 10-d-old light-grown seedlings of transgenic plants grown on MS media with 25 µg/mL hygromycin to select against segregants lacking the PGX1pro:PGX1-GFP transgene.

(B) Quantification of hypocotyl length in 6-d-old etiolated seedlings of Col and complementation lines grown on MS media without Suc. Bars indicate sd (n ≥ 40 seedlings from two independent experiments). Lowercase letters in (B) indicate significantly different groups (P < 0.05, one-way ANOVA and Tukey test).