Table 1. Monosaccharide Composition of Wild-Type and PGX1 Mutant Cell Walls.
Tissue | Genotype | Fuc | Rhamnose | Ara | Gal | Glc | Xyl | GalA | GlcA |
---|---|---|---|---|---|---|---|---|---|
ES | Col | 5.74 ± 0.42 | 5.37 ± 0.90 | 35.33 ± 3.10 | 16.38 ± 1.02 | 5.55 ± 0.91 | 8.61 ± 0.59 | 23.03 ± 2.98 | 3.73 ± 0.59 |
PGX1AT | 6.81 ± 0.75 | 5.81 ± 0.64 | 32.80 ± 1.35 | 16.96 ± 1.09 | 6.01 ± 0.60 | 8.49 ± 0.66 | 22.02 ± 2.67 | 3.34 ± 0.98 | |
PGX1OE-1 | 6.88 ± 0.55 | 5.72 ± 0.45 | 34.69 ± 3.22 | 18.15 ± 1.11 | 5.10 ± 0.62 | 8.42 ± 0.22 | 19.29 ± 2.28 | 3.51 ± 0.70 | |
PGX1OE-48 | 7.14 ± 0.42 | 5.27 ± 0.65 | 30.76 ± 2.05 | 21.33 ± 2.74 | 5.92 ± 0.69 | 8.80 ± 0.70 | 19.07 ± 2.72 | 3.40 ± 0.66 | |
pgx1-1 | 6.33 ± 0.55 | 4.69 ± 0.59 | 33.97 ± 1.87 | 16.60 ± 1.07 | 5.60 ± 0.76 | 8.99 ± 0.67 | 23.82 ± 4.07 | 0.54 ± 0.20 | |
pgx1-2 | 6.56 ± 0.59 | 5.77 ± 1.38 | 30.39 ± 1.79 | 19.69 ± 1.38 | 6.10 ± 0.21 | 7.83 ± 0.39 | 22.36 ± 3.16 | 2.60 ± 0.93 | |
L | Col | 2.84 ± 0.56 | 1.93 ± 0.15 | 8.01 ± 0.50 | 2.67 ± 0.11 | 50.10 ± 1.51 | 4.00 ± 0.20 | 30.41 ± 2.05 | |
PGX1AT | 2.77 ± 0.32 | 1.43 ± 0.06 | 5.94 ± 0.25 | 1.97 ± 0.12 | 53.90 ± 3.71 | 3.23 ± 0.05 | 30.79 ± 3.90 | ||
PGX1OE-1 | 2.50 ± 0.77 | 1.53 ± 0.11 | 5.57 ± 0.23 | 2.32 ± 0.18 | 67.80 ± 1.00 | 4.16 ± 0.42 | 16.14 ± 1.40 | ||
PGX1OE-48 | 2.93 ± 0.21 | 1.66 ± 0.04 | 6.04 ± 0.20 | 2.58 ± 0.14 | 61.10 ± 1.49 | 3.38 ± 0.10 | 22.33 ± 1.78 | ||
pgx1-1 | 2.24 ± 0.22 | 1.16 ± 0.05 | 4.84 ± 0.39 | 1.46 ± 0.08 | 64.10 ± 1.74 | 3.39 ± 0.17 | 22.83 ± 1.87 | ||
pgx1-2 | 2.10 ± 0.29 | 1.26 ± 0.08 | 5.01 ± 0.44 | 1.57 ± 0.14 | 59.00 ± 6.63 | 3.33 ± 0.10 | 27.76 ± 7.35 |
Sugar composition (GalA, galacturonic acid; GlcA, glucuronic acid) from 6-d-old etiolated seedlings (ES) and leaves from 40-d-old plants (L) of the wild type, PGX1AT, PGX1OE-1, PGX1OE-48, pgx1-1, and pgx1-2 genotypes. Sugar amounts are presented as mean percentages ± sd (n = 6). Bold values indicate mutant glycosyl residue compositions that are at least ±15% and are significantly different (P < 0.05, t test) compared to the wild type for at least two overexpression and/or for both mutant lines.