The rbohH rbohJ Double Mutant Was Defective in Pollen Tube Tip
Growth.
(A) Schematic representation of the T-DNA insertions in
RbohH and RbohJ. Open triangles show the
sites of T-DNA insertions in the mutant together with deleted nucleotide
numbers. Closed boxes indicate the protein-coding exons.
rbohH-2 and rbohJ-1 mutants were in the
Ws background, and
rbohH-3, rbohJ-2, and
rbohJ-3 were in the Col-0 background.
(B) RT-PCR analysis of the full-length transcripts of
rbohH and RbohJ expressed in each mutant
flower. AP2 was used as a control. Two biological and
technical replicates were performed.
(C) Dissected siliques harvested ∼7 d after hand
pollination. White arrowheads indicate unfertilized ovules. ♂ indicates
the genotype of the pollen grain, and ♀ indicates the genotype of the
pistil. Bar = 1 mm.
(D) Percentage of normal seeds, aborted seeds, and unfertilized
ovules in siliques. Siliques were harvested 5 to 10 d after hand pollination.
Closed bars indicate normal seeds; gray bars indicate aborted seeds; open bars
indicate unfertilized ovules. Values are means ± se
(n = 5 to 6 siliques); *P < 0.01
(Student’s t test).
(E) Length of pollen tubes germinated in vitro. Values are means
± sd (n = 11–30); *P < 0.01
(Student’s t test).
(F) Pollen tubes were visualized by aniline blue staining in vivo.
Pistils were harvested 12 h after hand pollination. Bar = 500 μm.