Figure 2.

The rbohH rbohJ Double Mutant Was Defective in Pollen Tube Tip Growth.

(A) Schematic representation of the T-DNA insertions in RbohH and RbohJ. Open triangles show the sites of T-DNA insertions in the mutant together with deleted nucleotide numbers. Closed boxes indicate the protein-coding exons. rbohH-2 and rbohJ-1 mutants were in the Ws background, and rbohH-3, rbohJ-2, and rbohJ-3 were in the Col-0 background.

(B) RT-PCR analysis of the full-length transcripts of rbohH and RbohJ expressed in each mutant flower. AP2 was used as a control. Two biological and technical replicates were performed.

(C) Dissected siliques harvested ∼7 d after hand pollination. White arrowheads indicate unfertilized ovules. ♂ indicates the genotype of the pollen grain, and ♀ indicates the genotype of the pistil. Bar = 1 mm.

(D) Percentage of normal seeds, aborted seeds, and unfertilized ovules in siliques. Siliques were harvested 5 to 10 d after hand pollination. Closed bars indicate normal seeds; gray bars indicate aborted seeds; open bars indicate unfertilized ovules. Values are means ± se (n = 5 to 6 siliques); *P < 0.01 (Student’s t test).

(E) Length of pollen tubes germinated in vitro. Values are means ± sd (n = 11–30); *P < 0.01 (Student’s t test).

(F) Pollen tubes were visualized by aniline blue staining in vivo. Pistils were harvested 12 h after hand pollination. Bar = 500 μm.