Detection of ROS on Stigmatic Papilla
Cells.
Detection of ROS is shown on stigmatic
papilla cells with Oxyburst Green 20 min after pollination.
(A) to (D) Intense fluorescence of Oxyburst Green
around a wild-type pollen tube growing within the stigmatic papilla cell
wall.
(E) to (H) Faint fluorescence of Oxyburst Green
around the rbohH-3 rbohJ-2 double mutant pollen tube growing
within the stigmatic papilla cell wall.
(A) and (E) show fluorescence images;
(B) and (F) show pseudocolor fluorescence images;
(C) and (G) show bright-field images; and
(D) and (H) show overlay images. Bar = 10
μm.