Figure 2.

Callus Formation Resembles Adventitious Root Formation.

(A) to (C) Phenotypes of the 8-DAC leaf explants on B5 medium containing 1 μM (A), 0.1 μM (B), and 0.01 μM (C) IAA.

(D) and (E) Leaf explants of alf4-1 (D) and clf-50 swn-1 (E) mutants, which are defective in adventitious root formation. A total of 30 leaf explants from each mutant were analyzed, and the results were consistent.

(F) and (G) Lateral root formation differs between wild-type Col-0 (F) and arf7-1 arf19-1 (G). Seedlings were grown on half-strength MS medium for14 d.

(H) and (I) Leaf explants from the arf7-1 arf19-1 double mutant on B5 medium (H) or B5 medium containing 1 μm IAA (I). Note that seedlings of the arf7-1 arf19-1 double mutant produce no lateral roots but can generate adventitious roots normally without IAA or callus with 1 μM IAA. A total of 30 leaf explants were tested, and the results were consistent.

Bars = 1 mm.