Figure 5.

Relative Expression of Genes Involved in the BL Signaling Pathway in the CRY1a/b RNAi and Null Segregant Embryos.

Reverse-transcribed mRNA from embryos from partially after-ripened grains imbibed in darkness or under BL for different durations was tested using gene-specific primers for transcript abundance of ELIP90 (A), ELIP58 (B), HY5 (C), or CIP8 (D). Each point is the average ± se of relative transcript abundances from three biological replicates of 15 embryos each. Statistically significant differences between RNAi lines and null segregants using Dunnett’s two-tailed test at α = 0.05 are indicated with an asterisk.