Figure 4.

Double Mutations in GL3 and EGL3 Block Trichome Initiation in the penta Mutant.

(A) Images of the fifth true leaves of penta and penta gl3 egl3 heptuple mutant treated without (Mock) or with GA₃ (GA).

(B) Statistical analysis of total trichome numbers per leaf indicated in (A). Eight leaves for each genotype were used for trichome number measurement in each biological experiment. Data are means (±se) of three biological replicates.

(C) and (D) Real-time PCR analysis for GL2 (C) and MYB23 (D) in penta and penta gl3 egl3 mutants treated without (Mock) or with GA₃ (GA). ACTIN8 was used as the internal control. Data are means (±se) of three biological replicates. Lowercase letters indicate significant differences by one-way ANOVA analysis with SAS software (P < 0.05).

[See online article for color version of this figure.]