Figure 6.

RGA and JAZ1 Coordinately Inhibit Transcriptional Function of EGL3 and GL1.

(A) Schematic diagram of the constructs used in transient expression assays in (B) and (C).

(B) and (C) Transient expression assay showed that both RGA and JAZ1 proteins could significantly repress transcriptional function of EGL3 (B) and GL1 (C). The GUS/LUC ratios were measured in Arabidopsis leaf protoplasts after transiently transfected with reporter, internal control, and indicated effectors. Data are means (±se) of three biological replicates. Asterisks represent Student’s t test significance between pairs indicated with brackets (*P < 0.05; **P < 0.01).