Figure 3.

Analysis of ROS in the Wild Type and hhl1 Mutants.

(A) DAB staining of H₂O₂ in 3-week-old wild-type (Col-0), hhl1-1, and hhl1-2 plants under growth light (top panel) and after growing under high light for 2 d (bottom panel). GL, growth light; HL, 2-d high-light treatment.

(B) NBT staining of O₂⁻ in 3-week-old wild-type (Col-0), hhl1-1, and hhl1-2 plants under growth light (top panel) and after growing under high light for 2 d (bottom panel).

(C) Detection of ¹O₂ with SOSG in wild-type (Col-0), hhl1-1, and hhl1-2 plants under growth light and after growing under high light for 2 d.

(D) Detection of total ROS with DCF in wild-type (Col-0), hhl1-1, and hhl1-2 plants under growth light and after growing under high light for 2 d. Five additional independent biological replicates were performed, and similar results were obtained.

[See online article for color version of this figure.]