Figure 1.

Root-Tip Metaphases with Graphically Presented Chromosomal Types.

(A) and (C) to (I) Double-target FISH with 45S (green) and 5S rDNA (red, arrowed) as probes.

(A) to (E) Bivalent forming species.

(A) and (B) Oe. elata ssp hookeri strain hookeri de Vries.

(B) DAPI gray-scale image (left) of the metaphase plate shown in (A) and its graphical interpretation (right); arrowheads indicate secondary constrictions.

(C) Oe. elata ssp hookeri strain johansen Standard.

(D) Oe. grandiflora strain grandiflora Tuscaloosa.

(E) Oe. glazioviana strain blandina de Vries.

(F) to (I) Species that exhibit rings at meiosis.

(F) Oe. biennis strain suaveolens Grado.

(G) Oe. biennis strain suaveolens Standard.

(H) Oe. villosa ssp villosa strain bauri Standard.

(I) Oe. glazioviana strain r/r-lamarckiana Sweden.

I to VII, a to n, chromosome types distinguished on the basis of statistical calculations, i.e., their lengths and arm ratios are means from Supplemental Tables 2 and 3. Note that this designation does not imply chromosome homology/homoeology between strain or species. Single asterisks, chromosomal markers distinguished by their morphology and arrangement of rDNA loci; double asterisks, chromosomal markers based exclusively on morphology; arrowhead in I indicates the centromere of the chromosome “g” of Oe. glazioviana strain r/r-lamarckiana Sweden. Bars = 5 μm.