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. 2014 Mar 4;26(3):1294–1307. doi: 10.1105/tpc.113.116244

Figure 6.

Figure 6.

Reduced IRT1 Protein Amounts in the Absence of SNX Proteins.

(A) Immunoblot analysis of IRT1 protein abundance in roots of Col-0, snx1-2, and snx triple plants grown in the 2-week system. Iron-sufficient (50 μM Fe), iron-deficient (0 μM Fe), and resupply (res) conditions are indicated. Three independent experiments were performed, showing similar results.

(B) Quantification of (A) based on three independent experiments. Bars represent normalized protein abundance under iron-sufficient (50 μM Fe; black), iron-deficient (0 μM Fe; light gray), and resupply (dark gray) conditions. Error bars represent sd. Asterisks indicate statistically significant decrease of IRT1 signal in snx1-2 and snx triple, in comparison to Col-0, under iron-deficient conditions (P < 0.01).

(C) Immunoblot analysis of IRT1 protein abundance in roots of irt1-1, Col-0, snx1-1, and SNX1pro:SNX1-GFP/snx1-1 (labeled as SNX1-GFP) plants grown in the 2-week system and then only under iron deficiency. Three independent experiments were performed, showing similar results.

(D) Quantification of (C) based on three independent experiments. Bars represent normalized protein abundance. Error bars represent sd. Asterisks indicate statistically significant decrease of IRT1 signal in irt1-1 and snx1-1, in comparison to Col-0, under iron-deficient conditions (P < 0.005).