Figure 1.

Vacuolar Sorting Does Not Bypass the Golgi Apparatus.

(A) Schematic illustration of the recombinant proteins studied and summary of subcellular localization patterns. SP, signal peptide; Aleurain, propeptide of barley aleurain containing the vacuolar sorting signal.

(B) Confocal laser scanning micrographs of Agrobacterium-infiltrated tobacco leaf epidermis cells expressing secRFP, Aleu-RFP, RFP-HDEL, or Aleu-RFP-HDEL. Bars = 5 μm.

(C) As in (B), but micrographs were taken at lower magnification. Bars = 5 μm.