Expression of PexRD2 Promotes in Planta Growth and Sporulation of P.
infestans.
(A) Transient expression of GFPPexRD2 in one half of a
N. benthamiana leaf by agroinfiltration, followed by
inoculation with P. infestans 88069 zoospores, enhances the
growth of the pathogen, compared with expression of GFP in the second half.
Image taken at 5 d after zoospore inoculation.
(B) Lesion area achieved at 5 d after zoospore inoculation
following infection of GFP- or GFPPexRD2-expressing leaf tissue with
P. infestans 88069. Results are the mean ±
se of infections from two independent experiments using at least
eight leaves each. The asterisk indicates a value significantly different from
the GFP control (P < 0.001) as determined by t test.
(C) Percentage of total inoculation sites showing necrosis and/or
sporulation for the same infections.