Figure 2.

PexRD2 Interacts with the Kinase Domain of MAPKKKε.

(A) Y2H assay showing that PexRD2 interacts with the kinase domain (KD) of St-MAPKKKε, as evidenced by growth on selective media lacking His (-HIS) and the development of blue coloration in the presence of X-Gal. No interaction was detected between PexRD2 and a second MAPKKK, Sl-MAPKKKα.

(B) The N-terminal region of PexRD2 (residues 21 to 56, containing the translocation motifs) is not required for the interaction with StMAPKKKε-KD. Furthermore, the two PexRD2-like proteins, PexRD2-like-1a and PexRD2-like-2a, do not interact with StMAPKKKKε-KD. The symbols + and – denote interaction and absence of interaction, respectively.