Figure 5.

PexRD2 Variants Showing Perturbed Interactions with MAPKKKε, or PexRD2-Like Effectors, Fail to Enhance Pathogen Growth.

Transient expression of wild-type ^GFPPexRD2 in one half of a N. benthamiana leaf by agroinfiltration, followed by inoculation with P. infestans 88069 zoospores, enhances the lesion area achieved at 5 d after zoospore inoculation, relative to the GFP control. A similar level of enhancement was observed for PexRD2^Lys104Glu. No significant enhancement of pathogen growth was observed for the four variants of PexRD2 that show perturbed interactions with MAPKKKε (Leu109Asp, Leu112Asp, PexRD2^hepta, or PexRD2^octa) or the two PexRD2-like effectors. Results are the mean ± se of infections from two independent experiments using at least eight leaves each time. The asterisks indicate values significantly different from the GFP control (P < 0.01) as determined by one-way ANOVA; all other values were not significantly different (P > 0.05).