Figure 7.

MAPKKKε-Triggered Cell Death Is Suppressed by PexRD2.

Coexpression of ^GFPPexRD2 with SlMAKKKε-KD in N. benthamiana reduces the percentage of infiltration sites with >50% confluent cell death at 2 to 7 d post-β-estradiol treatment (dpt) compared with coexpression with the GFP control (A). By contrast, PexRD2 did not suppress the cell death triggered by either the kinase domain of SlMAPKKKα-KD (B) or a constitutively active mutant of the proposed downstream MAPKK, SlMEK2^Asp-Asp (C). Results are the mean ± se of three independent experiments, each using at least three plants. Asterisks indicate values significantly different from the GFP control at the same time point (P < 0.01) as determined by one-way ANOVA; all other values were not significantly different (ns; P > 0.05). Images indicate representative infiltration sites at 7 dpt.