Figure 2.

PawS1 Proteins from the Daisy Family (Asteraceae) and Their Buried Peptides

(A) PawS1 domains include the ER signal, PDP region, and the small (SSU) and large subunits (LSU) of mature albumin based on sunflower PawS1 (Mylne et al., 2011).

(B) Percentage (0 to 100%) identity graph for 28 PawS1 protein sequences showing weaker conservation around the PDP region (dashed box). The arrowhead beneath the graph indicates the position where four PawS1 sequences have 100 amino acid internal expansions. See Supplemental Figure 5 for an alignment of full-length sequences.

(C) Alignment of selected in vivo confirmed (closed circles) and gene-predicted (open circles) PDPs showing little conservation except the N-terminal Gly, C-terminal Asp, a Cys-pair, and central Pro-Pro. The BBI-loop mimics are marked with asterisks.

(D) Phylogenetic tree (Supplemental Data Sets 3 and 4) including species used in this study and showing PDPs are common to the Heliantheae and Millerieae tribes estimated to have diverged (dashed circle) 18 million years ago (Ma) (Supplemental Figure 6). Tribe names are black, whereas subtribe names are in red/gray.

[See online article for color version of this figure.]